Changes in diversity, composition and assembly processes of soil microbial communities during Robinia pseudoacacia L. restoration on the Loess Plateau, China

Robinia pseudoacacia L. (RP) restoration has increased vegetation cover in semi-arid regions on the Loess Plateau of China, but ecological problems have also occurred due to RP restoration, such as reduced soil moisture. Further, it is still uncertain how microbial diversity, composition and assembly processes change with regions. Therefore, amplicon sequencing small subunit ribosomal ribonucleic acid (16S rRNA) internal transcribed spacer (ITS) genes was performed study bacterial fungal at four sites different stand ages plantations (Y10, plantation less than 10 a; Y15, approximately 15 Y25, 25 Y40, 40 a) along a 40-a chronosequence Plateau. The diversity bacteria fungi significantly during period from (P<0.05). However, compared lower Y25 remained stable between a. relative abundances Proteobacteria Ascomycota Conversely, after they both decreased, whereas Actinomycetes, Acidobacteria Basidiomycota gradually increased. variations communities were mainly related changes total nitrogen, nitrate nitrogen moisture contents, while shaped by organic carbon contents. Bacterial structured heterogeneous selection stochastic process, primarily process. induced an increase importance communities. Overall, this reveals community provides new perspective effects